Does it matter which PCIe slot I put my graphics card in?

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Depending on your graphics card and motherboard, some of the PCI-e slots on your motherboard will be specifically meant for use with your primary graphics card  EXPLAINED: PCIe lane SWITCHING & Biggest MISTAKES!

slot pierino For example, an x1 slot can have only one transfer lane used by the x1 card inserted into the slot; x2, x4, x8, and x16 slots are also available Slot power · x1 cards are limited to A at +12 V and 10 W combined · x4 and wider cards are limited to A at +12 V and 25 W combined · A full-